Hi, I have one stupid question. The coordinates of the region chr1 2351533 - 2351843 from UCSC (hg18) will retrieve 311 bases. However, when I use Fetch Sequences from galaxy, it will only retrieves 310 bases. Apparently, the first base of the 311 bases is missing from the Fetch Sequences result because the ending bases are the same.
Does this mean that I need to modified the coordinates first and then use the Fetch Sequences to get the correct sequence? I thought UCSC and galaxy were both 0 base? Thanks. Sean
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