Hi, I used the Megablast function (in the NGS: Mapping\ROCHE-454\) to analyze my FASTA sequences against nt database and it worked fine for me. However, it generated 56,804 hits although my query has only 1000 sequences. I am wondering is there any way to suppress the number of reported alignments to just one best hit per sequence? (In the local BLAST there are parameters such as -K1 -v 1 -b 1 to do so, but I can't find similar options in Galaxy).
Many thanks! Sam ___________________________________________________________ The Galaxy User list should be used for the discussion of Galaxy analysis and other features on the public server at usegalaxy.org. Please keep all replies on the list by using "reply all" in your mail client. For discussion of local Galaxy instances and the Galaxy source code, please use the Galaxy Development list: http://lists.bx.psu.edu/listinfo/galaxy-dev To manage your subscriptions to this and other Galaxy lists, please use the interface at: http://lists.bx.psu.edu/