Hi galaxy dev team:

 

I just installed galaxy on my ubuntu box and tried to do RNA-seq analysis
according to Jeremy's excellent tutorial

 

http://main.g2.bx.psu.edu/u/jeremy/p/galaxy-rna-seq-analysis-exercise

 

however, I encountered following error messages when I was trying to execute
Cufflinks in galaxy..

 

Dataset generation errors

Dataset 121: Cufflinks on data 92: gene expression

Tool execution generated the following error message:

Error running cufflinks. [Errno 2] No such file or directory:
'transcripts.gtf'

The tool produced the following additional output:

cufflinks v1.0.3

cufflinks -q --no-update-check -I 300000 -F 0.050000 -j 0.050000 -p 4 -b 

 

how should I solve this problem?

 

Thanks in advance.

 

 

Best Regards,

 

John Wu

 

___________________________________________________________
The Galaxy User list should be used for the discussion of
Galaxy analysis and other features on the public server
at usegalaxy.org.  Please keep all replies on the list by
using "reply all" in your mail client.  For discussion of
local Galaxy instances and the Galaxy source code, please
use the Galaxy Development list:

  http://lists.bx.psu.edu/listinfo/galaxy-dev

To manage your subscriptions to this and other Galaxy lists,
please use the interface at:

  http://lists.bx.psu.edu/

Reply via email to