I'm reposting this question to the galaxy-user mailing list.
> Dear Anton,
> I am a PhD student in the Matrin-Luther University Halle-Wittenberg focused
> on honey bee Ecology.
> Galaxy is a very flexible tool to do RNA Seq analysis. I have a problem and
> need your help. The galaxy provide build in annotated reference genome of
> honey bee (2005). This is a old version and the lastest version (2011) has
> much higher resolution
> (http://www.ncbi.nlm.nih.gov/nuccore?term=CM000054:CM000069[PACC]). I tried
> to bulid a customized annotated reference genome. First, I upload the
> annotated reference genome (.gb file), but can not choose it when I map the
> read (Tophat for Illumina). Then I upload the reference genome without
> annotation (.fasta file), it works. But I need the annotation of the genes to
> analyze the differencial expression.
> We are very interested in galaxy and use it in our research analysis. Hope
> you can help us solve this problem.
> Expecting your reply!
> All the best,
> Qiang Huang
> Institut für Biology/Zoologie, Molekulare Ökologie, Martin-Luther-Universität
> Halle -Wittenberg
> 06099, Halle, Germany.
> Honeybee Research Institute, Jiangxi Agricultural University, 330045,
> Nanchang, China.
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