Hi,
I currently have read names with the format:
@N57638:1:64JU0AAXX:1:1:1057:943 1:Y:0:
and would like to change them to the format:
@N57638:1:64JU0AAXX:1:1:1057:943/1
I use Manipulate FASTQ, on all reads and set 'Manipulate Reads on:' to
'Name/Identifier', ('String Translate' becomes the only option).
I then set the 'From:' field to '1:Y:0:' and the 'To:' field to '/1' (without
the literal quotes).
I get the following error:
Traceback (most recent call last):
File
"/home/home/galaxy/software/galaxy-central/tools/fastq/fastq_manipulation.py",
line 37, in
main()
File
"/home/home/galaxy/software/galaxy-central/tools/fastq/fastq_manipulation.py",
line 25, in main
new_read = fastq_manipulator.match_and_manipulate_read( fastq_read )
File
"/home/home/galaxy/software/galaxy-central/database/job_working_directory/942/tmpgp13Qy",
line 15, in match_and_manipulate_read
new_read = manipulate_read( fastq_read )
File
"/home/home/galaxy/software/galaxy-central/database/job_working_directory/942/tmpgp13Qy",
line 8, in manipulate_read
new_read.identifier = "@%s" % new_read.identifier[1:].translate( maketrans(
binascii.unhexlify( "313a593a303a" ), binascii.unhexlify( "2f31" ) ) )
ValueError: maketrans arguments must have same length
So, do the From and To fields really need to be the same length?
This seems rather strange and unhelpful.
Am I doing something wrong?
Many thanks,
Graham
Dr. Graham Etherington
Bioinformatics Support Officer,
The Sainsbury Laboratory,
Norwich Research Park,
Norwich NR4 7UH.
UK
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