Hello Tracy,
In the group "NGS: QC and manipulation -> FASTX-Toolkit for FASTQ data"
there are two tools "Barcode Splitter" and "Trim sequences". Use the
first tool, then the second. Instructions are on each tool's form and
details are at the FASTX-Toolkit web site (link is also on the tool form).
Hopefully this helps to get you started, please let us know if you have
any question.
Thanks for using Galaxy!
Jen
Galaxy team
On 10/17/11 5:39 AM, Qingquan Liu wrote:
Hi, I have an Illumina HiSeq lane of sequences, in which I input
multiple samples with 5-prime 6 bp barcodes. The barcodes were added in
a way so that the barcodes are the first 6 bp in the reads. What I need
to do is to sort my reads according to the barcodes, then clip off the
barcodes and use the remaining to do mapping. Could anybody advise how I
could sort my reads based on the barcodes (e.g. the first 6 base pairs)
in Galaxy? Thanks a lot!
Tracy
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___________________________________________________________
The Galaxy User list should be used for the discussion of
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at usegalaxy.org. Please keep all replies on the list by
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