Hello Tracy,

In the group "NGS: QC and manipulation -> FASTX-Toolkit for FASTQ data" there are two tools "Barcode Splitter" and "Trim sequences". Use the first tool, then the second. Instructions are on each tool's form and details are at the FASTX-Toolkit web site (link is also on the tool form).


Hopefully this helps to get you started, please let us know if you have any question.

Thanks for using Galaxy!

Jen
Galaxy team


On 10/17/11 5:39 AM, Qingquan Liu wrote:
Hi, I have an Illumina HiSeq lane of sequences, in which I input
multiple samples with 5-prime 6 bp barcodes. The barcodes were added in
a way so that the barcodes are the first 6 bp in the reads. What I need
to do is to sort my reads according to the barcodes, then clip off the
barcodes and use the remaining to do mapping. Could anybody advise how I
could sort my reads based on the barcodes (e.g. the first 6 base pairs)
in Galaxy? Thanks a lot!

Tracy



___________________________________________________________
The Galaxy User list should be used for the discussion of
Galaxy analysis and other features on the public server
at usegalaxy.org.  Please keep all replies on the list by
using "reply all" in your mail client.  For discussion of
local Galaxy instances and the Galaxy source code, please
use the Galaxy Development list:

   http://lists.bx.psu.edu/listinfo/galaxy-dev

To manage your subscriptions to this and other Galaxy lists,
please use the interface at:

   http://lists.bx.psu.edu/

--
Jennifer Jackson
http://usegalaxy.org
http://galaxyproject.org/wiki/Support
___________________________________________________________
The Galaxy User list should be used for the discussion of
Galaxy analysis and other features on the public server
at usegalaxy.org.  Please keep all replies on the list by
using "reply all" in your mail client.  For discussion of
local Galaxy instances and the Galaxy source code, please
use the Galaxy Development list:

 http://lists.bx.psu.edu/listinfo/galaxy-dev

To manage your subscriptions to this and other Galaxy lists,
please use the interface at:

 http://lists.bx.psu.edu/

Reply via email to