We are just beginning to analyze RNA-seq data and hope to make use of the
Galaxy tools. We are not using a commonly studied organism such as human.
Rather, our data are from the bacterium Pseudomonas fluorescens Pf0-1.
The genome for P. fluorescens Pf0-1 appears in the "database/build" menu,
and we have selected this in the .sam file attributes.
When we run the sam to bam conversion, we get the error "Sequences are not
currently available for the specified build."
Is this something that can be corrected easily?
Many thanks for your help.
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