I am trying to extract sequences from a FASTA file containing genomic
information. The coordinates are in a tab-delimited format, which is recognized
as BED format by Galaxy (meaning that the 6th column is correctly interpreted
as 6. Strand).
However, upon running "Fetch sequences" , Extract Genomic DNA only the +-strand
information is included in the output FASTA file and I receive the following
format: fasta, database: ?
Info: 1476 warnings, 1st is: Invalid interval, start '1616' > end '1177'.
Skipped 1476 invalid lines, 1st is #2, "scaffold00001 1616 1177 Fom -
Is this a bug? How can I can adjust my input data files to get the --strand
sequences as well?
I have seen a similar problem in an earlier posting and there it was suggested
to manually adjust the strand information column 5, but this did not work for
Many thanks for your all help!!!!!
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