David, in my experience with Illumina sequencing, it looks like the
reads at the start of a file have a much higher sequencing error rate.
On Nov 9, 2011, at 4:52 AM, David Matthews wrote:
This may be a bit dumb or missing the point but just selecting the
first 5 million is kind of random isn't it? I mean where the reads
map and what they are from is not known to you and they were not
collected by the sequencer in a manner that is influenced by the
nature of the sample?
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