to the best of my knowledge reads at the start of SOLiD data set also have a higher error rate .. I think it might be also due to edge effect.
On 9 November 2011 21:49, Peter Cock <p.j.a.c...@googlemail.com> wrote: > On Wed, Nov 9, 2011 at 12:03 PM, Bob Harris <rshar...@bx.psu.edu> wrote: > > David, in my experience with Illumina sequencing, it looks like the > reads at > > the start of a file have a much higher sequencing error rate. > > Bob H > > Yes, reads at the start and the end of the file come from the edge of > the Illumina slide, and tend to be of poorer quality that the reads from > the middle. > >
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