Hello Aman,

The quickest way to use new genomes with tools is to use them as a custom reference genome. To do this, load the genome in fasta format into your history and set the tool form option to use the reference genome "from the history".


Best practices:
1 - double check that the format is strict fasta (all lines wrapped to same length, no extra lines or hidden characters, sequence identifiers are unique).
  2 - use the same genome dataset for all steps within an analysis
  3 - load using FTP and make certain load is complete
      http://galaxyproject.org/wiki/Learn/Upload%20via%20FTP

Hopefully this helps!

Best,

Jen
Galaxy team

On 1/14/12 6:32 PM, Amandeep Sidhu wrote:
Hi All,

We need Vicugna Pacos (http://asia.ensembl.org/Vicugna_pacos/Info/Index) added 
as a reference genome to run analysis using Top Hat on the public server.

Thanks.

Regards,
Aman.

------------
Sent from Aman's iPad
@biomap | http://amandeep.org/ | http://biomap.org/

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