Hello Erin,

This was a temporary problem due to a new filesystem we installed during this time frame, that has since been resolved. Please try again and if the problem persists, please send a bug report from the error dataset using the green bug icon. This allows us to gain access to the inputs and job parameters to diagnose the root cause of the problem.


http://wiki.g2.bx.psu.edu/Support#Error_from_tools
http://wiki.g2.bx.psu.edu/Support#Unexpected_scientific_result

Thank you!

Jen
Galaxy team

On 1/20/12 6:51 AM, Erin Shanle wrote:
Hello
I am having a problem running Cuffdiff on some RNA-seq data.  I want to
compare 2 of my conditions.  I successfully used Cuffdiff three days ago
to compare two sets of data that are processed the exact same way (align
with Tophat and use Picard to confirm adequate alignment). I am using
Galaxy through MAIN and I tried Cuffdiff with these samples on 1/18 and
1/19.  Here's the error:

An error occurred running this job: /cuffdiff v1.3.0 (3022)
cuffdiff --no-update-check -q -p 8 -c 1000 --FDR 0.050000 -b
/galaxy/data/hg19/sam_index/hg19.fa --labels DMSO,E2
/galaxy/main_pool/pool2/files/003/607/dataset_3607369.dat
/galaxy/main_pool/pool2/files/003/590/dataset_3590726.dat,/g/

Thanks for the help.
--
Erin Shanle

Graduate Research Assistant
Molecular and Environmental Toxicology
425 McArdle Laboratory for Cancer Research
1400 University Ave
Madison, WI 53706
608 262 9834
sha...@wisc.edu <mailto:sha...@wisc.edu>


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___________________________________________________________
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