Hi Benoit,

The best person to ask about parameters is probably the tool author.

The wiki source has some comments, but not in the detail you are looking for: http://code.google.com/p/ea-utils/wiki/FastqMcf

It looks like the author is answering questions at http://seqanswers.com. A search on "fastq-mcf" found four threads, the largest one with quite a but of parameter clarification in it. This would be a good place to post a new question question about any parameters not covered in the other threads.

Hopefully this helps,

Best,

Jen
Galaxy team

On 3/2/12 12:25 PM, BenoƮt REMENANT wrote:
Hi all,
Does someone know fastq-MCF?
We are relatively newbies in analysing RNAseq results, and we would like
to use the fastq-MCF program to trim our illumina sequences (based on
quality score and adapters sequences) while keeping paired reads
synchronized. Unfortunately, there are some parameters we did not
understand, like -s (log scale for clip pct to threshold) and -t (%
occurrence threshold before clipping).
Does anyone can explain us what these parameters mean? Or when I can
found useful information?
And do you think we have to unable the PF filtering (and why)?
Thank you very much in advance if someone can help us.
Benoit


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