Hello all, I am certainly new to using galaxy and I have already checked the message boards to get some assistance on this but was unsuccessful. Anyways, here is my problem, I have a a FASTA file of C. gigas partial sequences. I was trying to use Galaxy's equicktandem tool to identify micro satellites in the sequence. However, when I ran the tool it did not yield any results. This is strange because I could visually pick out where l thought the algorithm should have found a hit. Additionally, trimming my collection of sequences to a single sequence did not yield any hits. Moreover, I created a fake sequence with very obvious "microsatellites" and the algorithm appeared to work fine as it identified the repeats. While I suppose it is certainly possible that there may just not be any microsatellites in my sequence, I find it highly unlikely and suspect something else is going on. This seemed like such a simple tool yet I am having much difficulty in using it. I am simply uploading my FASTA file normally and then taking that file and using the tool. I am doing this all online through the galaxy website not a local instance and if you would like to see yourself the FASTA file can be downloaded at http://genefish.wikispaces.com/crassostreome and the FASTA file is cgigas_alpha_v0.3.2 (the collection of 272 sequences). Please help this has been excruciatingly frustrating for me and I suspect that a veteran Galaxy user may be able to see my error quickly.
Best, Harry hpodsch...@gmail.com ___________________________________________________________ The Galaxy User list should be used for the discussion of Galaxy analysis and other features on the public server at usegalaxy.org. Please keep all replies on the list by using "reply all" in your mail client. For discussion of local Galaxy instances and the Galaxy source code, please use the Galaxy Development list: http://lists.bx.psu.edu/listinfo/galaxy-dev To manage your subscriptions to this and other Galaxy lists, please use the interface at: http://lists.bx.psu.edu/