Hi Jiwen,

As far as I know, this is possible. The CuffDiff log2 value is defined here: http://cufflinks.cbcb.umd.edu/manual.html#gene_exp_diff


7   FPKMx              8.01089   FPKM of the gene in sample x
8   FPKMy              8.551545  FPKM of the gene in sample y
9   log2(FPKMy/FPKMx)  0.06531   The (base 2) log of the fold
                                 change y/x

And log(2) in general (including a graph, which can help with visualizing) is described here (although the web is full if similar):
http://en.wikipedia.org/wiki/Logarithm

I can see that this same Q on seqanswers.com recieved pretty much the same answer (in brief! http://seqanswers.com/forums/showthread.php?t=19558), so I think you are safe using the data as it as generated. Taking a look at the inputs would be advised if the results were unexpected.

If you do still have concerns about the log(2) calculation, asking the tool authors directly (if you have't done so already) at tophat.cuffli...@gmail.com is always an option, to tripple check.

Best,

Jen
Galaxy team

On 4/26/12 7:48 AM, 杨继文 wrote:
Hi,
I am analyzing my RNA-Seq data. After running cuffdiff, I got a list of
differentially expressed transcrpts or genes.
As far as I know, log2 value = fold change. However, there are minus
values. Is this possible?? log2 value can not be minus. Did I miss
something??
Looking forward to your help.
Thanks in advance.
Best
Jiwen


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