Dear Galaxy users that also use Megan,

I hope you can help me combining Galaxy with Megan4. I followed the Galaxy 
metagenome  workflow (author aun1) on shotgun DNA 454 sequences of two samples 
that I would like to compare. The results  are two nice trees and tables with 
the lowest taxonomic ranks for the two samples.
Besides the taxonomy I am also interested in the functions and I would like to 
use Megan4 (with SEED and KEGG classification) for this purpose. I tried to 
import a Galaxy file (.tabular) with c1 =high quality segment name and c2-c13 
=blasthit results and a Galaxy fasta file with the high quality segment names 
and sequences. This does not work and I receive the message parsing failed, no 
reads found.
I am interested to know how you solved this issue and which files in the Galaxy 
metagenome workflow you use to import into Megan4.
Or maybe there is other software that you use to compare suits of functional 
genes in your metagenome datasets?


Judith van Bleijswijk
The Galaxy User list should be used for the discussion of
Galaxy analysis and other features on the public server
at  Please keep all replies on the list by
using "reply all" in your mail client.  For discussion of
local Galaxy instances and the Galaxy source code, please
use the Galaxy Development list:

To manage your subscriptions to this and other Galaxy lists,
please use the interface at:

Reply via email to