not sure but think that they need to be in fastqsanger format to be used in the 
splitter. You probably have to convert them first with the groomer. But since I 
am only finding out Galaxy myself, maybe wait for a more user with more 
experience to answer your question.


Bram Van den Bergh
Centre of Microbial and Plant Genetics
Department of Microbial and Molecular Systems, K.U.Leuven Kasteelpark Arenberg 
20 box 2460, B-3001 Heverlee
Phone: +32 16 32 16 31 (secretariat)
Fax.: +32 16 32 19 63
[] namens Du, Jianguang []
Verzonden: donderdag 9 augustus 2012 23:29
Onderwerp: [galaxy-user] how to split paired-end dataset of FASTQ format

I downloaded RNA-seq dataset at FASTQ format from SRA of NCBI. I uploaded the 
dataset onto Galaxy. The dataset is paired-end. I want to split it into two 
datasets (one for each end) with FASTQ splitter. But the name of the dataset 
does not appear under "FASTQ reads". How should I do to solve this problem?


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