Dear All,

I have some FASTQ datasets in phred 33 offset, and I have already assinged them 
Fastqsanger format. Do I need to run FASTQ Groomer on these datasets before I 
check the data quality by "Fastqc: Fastqc QC" and "FASTQ Trimmer by column" to 
remove bad nucleotides at 3' end of reads?

Should I select "Sanger" as "Input FASTQ quality scores type:" if I need to run 


Jianguang Du

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