Hi everyone,

I am Didier Zoccola and i am marine biologist working on corals.  I would like 
to do a illumina mapping from RNA-seq coming from environmental conditions. For 
that, i would like to use Bowtie but i have not a reference genome. We have 
done a de novo transcriptome using trinity assembly. I have filter by quality 
all my data. One possible thing is to download all the manipulated sequences on 
my computer and use  Bowtie on my machine but it will take long time because my 
computer is not appropriate for this. An other thing is to download my 
reference transcriptome on your server. Is it possible? 

If not, what is the characteristics of the "ideal" computer to do the job 
locally ?

thank you in advance 

Best regards

Didier Zoccola
Centre Scientifique de Monaco
avenue Saint Martin
98000 Monaco
☎ +377 97 77 08 73
http:// www.centrescientifique.mc

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