Hi, all!

I have a maybe naive question that might be not so related to Galaxy usage. So I got the RNA seq data from Illumina Hiseq. Do I need to get rid of the adapter manually? If so, is it "clip adapter" that I should use? It should only have one adapter sequence or maybe more than one? And the adapters should be in both sides or just one side?

Then my next question is from the Illumina sequencing principle, they use adapter to amplify sequence and primers to do base call, so sequence data should not have any adapter information, is it right?

In my case, my small RNA data are all 75bp sequences, which is weird to me because my expectation is a bundle of sequence ranging from 20-30bp. Have any of your guy encountered such problems?

Thanks in advance!

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