Hi Els,

try the tool 'Group data by a column and perform aggregate operation on
other columns.' And choose 'count' or 'count distinct.

Hope that will work.
Happy research!
Bjoern

> Dear all,
> 
>  
> 
> I am a Phd student working on chicken genomics, with limited
> experience in the bio-informatics field. I performed an RNA-Seq
> experiment with single end 50 bp reads to find differential gene
> expressions between different groups. I have mapped this data with
> Tophat. Does anyone know how I can get the raw data count out of
> galaxy in a tab-delimited file? I would like to perform a statistical
> analysis in other software, without the RPKM normalization that
> cufflinks offers.  Thank you very much.
> 
>  
> 
> Have a nice day.
> 
>  
> 
> Yours Sincerely, Els 
> 
>  
> 
> ---
> 
> Ir. Els Willems
> 
> KU Leuven
> 
> Department of Biosystems
> 
> Division Livestock - Nutrition - Quality
> 
> Laboratory of Livestock Physiology
> 
> Kasteelpark Arenberg 30 bus 2456
> 
> B - 3001 Heverlee
> 
> T (+32) 016 32 17 29
> 
> F (+32) 016 32 19 94
> 
>  
> 
> 
> ___________________________________________________________
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___________________________________________________________
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