I've searched the archives and cannot find my situation. I have an Illumina
MiSeq dataset with ~8 million reads that was aligned to the reference genome
on the MiSeq machine. (The machine uses BWA to allow overlapping reads,
unlike Casava.) The alignment generated dataset.bam and dataset.bam.bai
files in the same directlry.
I used Get Data to upload both files. The problems, possibly related, are
that Galaxy 1) would not recognize the .bam file as a bam file and 2) would
not recognize the dataset.bam.bai file as the bam file metadata.
After uploading the bam file, originally identified as a text file, I
manually used the pen option to tell Galaxy that the data was a .bam file. I
then uploaded the .bam.bai file. The data would not display the data at UCSC
genome browser nor in Trackster, even though those options were displayed
for the .bam file. After uploading, I also used the pen editor to change
from dataset.bam.bai to dataset.bai.
None of these options worked for Trackster, UCSC genome brower, nor IGV nor
IGB. Interestingly, IGV would display it if the data was used directly form
my hard drive but not through Galaxy.
Any advice would be greatly appreciated.
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