Hello Larry,
The "Manipulate Fastq" tool only brings up the regular trimmer tools
again once "sequence and trim" are selected, so this will not work. And
a regular expression could be used as a filter, but that will not
actually trim the data.
If you choose to filter, this regular expression would find sequences
with variable length poly-G at the end. This one actually finds "one or
more", so not really poly - this is for you to modify. Change the number
in the {} to make a minimum required length.
^.*[A|T|C|N]G{1}G*$
Are you trying to trim poly-A? If using a local instance, repeat masker
was just added to the Tool Shed and could be quicker. But if using the
public Main server, the adapter clip idea from Ido is very good -
certainly worth a try.
The other option is to just go ahead and align the data. If the region
is long for all sequences, or some subset (you could pull out those that
are very long), then do a blanket end length trim on all, put back
together any files you have split apart, and let the aligner deal with
the remaining trailing bases. "Manipulate Fastq" can be used to subset
the file - just run it twice (or as many times as needed to get all the
data uniquely into distinct files to merge later.
Best,
Jen
Galaxy team
On 7/28/13 11:44 AM, Larry Simpson wrote:
Hi
Is it possible to trim a variable number of a specific nucleotide from
the 3' ends of fastq RNA reads? The "Manipulate Fastq" utility in
Galaxy may have this ability but I do not know how to create a custom
inquiry.
Thanks in advance for any assistance.
Larry
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