Hi Yan,

I also had problems with NBIC FTP.
NBIC allows only 10 GB space for user.
I made my BAM files in main server (using Tophat2) and then uploaded them to 
NBIC using their download URLs.
It was fast. It took me less than a hour to move 16 BAM files (around 9.5 GB).
You may try this.
Good luck.

Best wishes,
Anto


________________________________
From: Yan He [yanh...@hotmail.com]
Sent: 22 August 2013 09:36
To: Anto Praveen Rajkumar Rajamani; galaxy-user@lists.bx.psu.edu
Subject: 答复: [galaxy-user] Counts of mapped reads for each gene?

Hi Anto,
Thank you very much for your reply! I tried Galaxy/NBIC. However, I had problem 
with uploading my files. I used FTP, because the file I had was larger than 2G, 
but I couldn’t connect to the NBIC FTP. Do you have some idea how to solve the 
problem? Thanks!
Yan

发件人: Anto Praveen Rajkumar Rajamani [mailto:a...@hum-gen.au.dk]
发送时间: Thursday, August 22, 2013 3:14 PM
收件人: Yan He; galaxy-user@lists.bx.psu.edu
主题: RE: [galaxy-user] Counts of mapped reads for each gene?

Hi Yan,

You may use the HTseq count wrapper in the http://galaxy.nbic.nl/.
It does a good job and I could employ edgeR on that count matrix.
Good luck.

Best wishes,
Anto


___________________________________________________________
The Galaxy User list should be used for the discussion of
Galaxy analysis and other features on the public server
at usegalaxy.org.  Please keep all replies on the list by
using "reply all" in your mail client.  For discussion of
local Galaxy instances and the Galaxy source code, please
use the Galaxy Development list:

  http://lists.bx.psu.edu/listinfo/galaxy-dev

To manage your subscriptions to this and other Galaxy lists,
please use the interface at:

  http://lists.bx.psu.edu/

To search Galaxy mailing lists use the unified search at:

  http://galaxyproject.org/search/mailinglists/

Reply via email to