Hello,

The easiest way to ensure that dependencies are set up correctly is to install tool dependencies directly from the Tool Shed:
http://wiki.galaxyproject.org/DefiningRepositoryDependencies#Complex_repository_dependencies:_tool_dependency

You will want to use:
http://toolshed.g2.bx.psu.edu/
"package_picard_1_56_0"

For local instance help, please post to the galaxy-...@bx.psu.edu mailing list, as that is the best way to reach the development community. I am going to double post this reply, to resolve the question on the original galaxy-user and to give it visibility on the galaxy-dev list, but going forward on this thread (if needed) please remove galaxy-user from future replies.

Best!

Jen
Galaxy team


On 8/31/13 11:19 AM, Nicolas Quizon wrote:
Dear Galaxy Gurus,

I am a student and still new to Galaxy. I am encountering an error when trying to run the Picard Paired Read Mate Fixer with default settings on Galaxy. The code only runs for a few seconds before failing and displaying the error message.

## exit code=1; stdout=
[Mon Aug 19 16:46:15 EDT 2013] net.sf.picard.sam.FixMateInformation INPUT=[/usr/lib/galaxy-server/database/files/000/dataset_85.dat] OUTPUT=/usr/lib/galaxy-server/database/tmp/tmpxhbz3T SORT_ORDER=coordinate VALIDATION_STRINGENCY=LENIENT VERBOSITY=INFO QUIET=false COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false [Mon Aug 19 16:46:15 EDT 2013] Executing as root@biolinux-pbcn6 on Linux 3.2.0-43-generic amd64; OpenJDK 64-Bit Server VM 1.6.0_27-b27; Picard version: 1.91() [Mon Aug 19 16:46:15 EDT 2013] net.sf.picard.sam.FixMateInformation done. Elapsed time: 0.00 minutes.
Runtime.totalMemory()=378470400
To get help, see http://picard.sourceforge.net/index.shtml#GettingHelp
Exception in thread "main" java.lang.NoClassDefFoundError: org/itadaki/bzip2/BZip2InputStream at net.sf.picard.sam.FixMateInformation.doWork(FixMateInformation.java:84) at net.sf.picard.cmdline.CommandLineProgram.instanceMain(CommandLineProgram.java:177) at net.sf.picard.cmdline.CommandLineProgram.instanceMainWithExit(CommandLineProgram.java:119) at net.sf.picard.sam.FixMateInformation.main(FixMateInformation.java:76) Caused by: java.lang.ClassNotFoundException: org.itadaki.bzip2.BZip2InputStream
    at java.net.URLClassLoader$1.run(URLClassLoader.java:217)
    at java.security.AccessController.doPrivileged(Native Method)
    at java.net.URLClassLoader.findClass(URLClassLoader.java:205)
    at java.lang.ClassLoader.loadClass(ClassLoader.java:321)
    at sun.misc.Launcher$AppClassLoader.loadClass(Launcher.java:294)
    at java.lang.ClassLoader.loadClass(ClassLoader.java:266)
    ... 4 more

For input I am using data that I have successfully mapped with BWA for Illumina. I currently have Python version 2.7.3 and Java version 1.6.0_27, and an working on a machine running Ubuntu 12.04.2 LTS.

Any help is greatly appreciated!

Thanks!


___________________________________________________________
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--
Jennifer Hillman-Jackson
http://galaxyproject.org

___________________________________________________________
The Galaxy User list should be used for the discussion of
Galaxy analysis and other features on the public server
at usegalaxy.org.  Please keep all replies on the list by
using "reply all" in your mail client.  For discussion of
local Galaxy instances and the Galaxy source code, please
use the Galaxy Development list:

  http://lists.bx.psu.edu/listinfo/galaxy-dev

To manage your subscriptions to this and other Galaxy lists,
please use the interface at:

  http://lists.bx.psu.edu/

To search Galaxy mailing lists use the unified search at:

  http://galaxyproject.org/search/mailinglists/

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