Dear all,

I am working on an MNAse-Seq experiment with 50bp single end reads. To identify nucleosome positions, I read that one needs to extend the single reads to approximately the length of nucleosome protected DNA, being approximately 150bp.

Is there a way in Galaxy to extend 50bp reads to 150bp length, lets say from a .BAM file with mapped reads?
Of course any other comment on this topic is much appreciated!

Thank you very much,


Tobias Hohenauer, PhD
GCNA, Disease Mechanism Research Core
RIKEN Brain Science Institute
2-1 Hirosawa, Wako-shi
351-0198 Japan

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