Dear all,

I am working on an MNAse-Seq experiment with 50bp single end reads. To identify nucleosome positions, I read that one needs to extend the single reads to approximately the length of nucleosome protected DNA, being approximately 150bp.


Is there a way in Galaxy to extend 50bp reads to 150bp length, lets say from a .BAM file with mapped reads?
Of course any other comment on this topic is much appreciated!

Thank you very much,

Tobias

--
Tobias Hohenauer, PhD
GCNA, Disease Mechanism Research Core
RIKEN Brain Science Institute
2-1 Hirosawa, Wako-shi
351-0198 Japan

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