On Wed, Nov 6, 2013 at 12:54 PM, Benjamin Osei-agyeman <
benjy_o...@yahoo.co.uk> wrote:

> The report was obtained after running fastqc on my data. However after
> looking at the report statistics, the
> Quality is not good in the Per Base Sequence Quality and there is a
> difference between the mean and median at each cycle. How can the quality
> be improved?

Is the quality poor across the entire read, or just at one end? You can
improve overall quality in two ways. By filtering out lower quality reads
(the Filter by Quality tool or Filter FASTQ tool) or by trimming a portion
of the reads (the FASTQ trimmer tool).

It is important to understand that these tools work by throwing away data,
you can't improve the overall quality of reads you already have. The FASTQC
tool is telling you about the quality of your sequencing experiment. The
only way to improve the overall quality is to do the experiment again.

> And why is there a difference between the mean and median in the fastqc
> report at each cycle?

It means your quality score distribution is skewed. This is not necessarily
a concern.

James Taylor, Associate Professor, Biology/CS, Emory University
The Galaxy User list should be used for the discussion of
Galaxy analysis and other features on the public server
at usegalaxy.org.  Please keep all replies on the list by
using "reply all" in your mail client.  For discussion of
local Galaxy instances and the Galaxy source code, please
use the Galaxy Development list:


To manage your subscriptions to this and other Galaxy lists,
please use the interface at:


To search Galaxy mailing lists use the unified search at:


Reply via email to