To whom it may concern

I do have a problem with tophat. I can easily put fastq data to "history" and according to RNA-seq Analysis Exercise provided by Jeremy. We checked the type of Ascii ofset for the quality estimation. I tried even "quality data converter" set to 33 (we do have data of this ASCII offset from 2 different sources) but "tophat for Illumina" simply can not read the data before and even after quality format converter. We do not have any idea what is going on. I am logged in Galaxy with current email, can you check my data and is there any converter for quality offset?

Miro Sotak
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