Hi Galaxy team,
I recently met two problems when I used " Fetch sequences> Extract Genomic
DNA" in Galaxy Main instance.
I wanted to extract the exons according to the coordinates in a GFF3 file from
my reference sequences (from history, and I specified the genome build) which
are in FASTA format.
But after checking the output, I found:
1. The first base of each extracted exon was missing in the output, so each
extracted exon sequence is one nucleotide shorter than the real length.
2. Some extracted exons are correct,but some extracted exons are wrong. The
questionable exons could not be found in the corresponding reference. I can not
figure out where they are from.
I tried to read the manual/warnings in the page. But I have no idea with my
strange output. Could anyone give me some clues,please?
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