I am attempting to megablast a FASTA file containing ~45,000 records of 100nt 
each against the nt database using the Galaxy web interface. I am using a 
Galaxy-generated FASTA file produced from a groomed FASTQ file using the 'FASTQ 
to tabular' and 'Tabular to FASTA' tools. When I try to use this as input for 
the Megablast tool, I get the "Required metadata values are missing" error. 
When I look at the attributes of the FASTA file, the datatype is indeed set to 
FASTA. I have tried downloading the FASTA file and reuploading it as a FASTA 
file explicitly with the same result. I also tried changing the FASTA width to 
79, and trimming the records to 70nt each – not the problem. If I make a file 
containing only the first FASTA record, I get the same error. Any suggestions?


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