I am analysing some RNA-seq data and have two different datasets, total RNA 
plus enzyme and total RNA minus enzyme. I used to type in "column join" in the 
"Tools" menu on the Galaxy homepage after uploaded the files in Text delimited 
format. Column join allowed me to join two datasets by a hinge column, in this 
case I would choose column 1 which would be the chromosome position. I would 
also be allowed to fill empty columns with a value of 1. The output would give 
me 3 columns, chromosome number (which both datasets shared), reads for minus 
enzyme, and reads for plus enzyme. The function would never reject any 
chromosome position with reads in one dataset and not the other. However, I 
cannot seem to be able to find this function anymore, and the new function 
"Join two datasets" seems to only give me back rows where both plus and minus 
datasets have reads. Does anyone else have this problem or know how I could get 
around it?



Justin Clarke
McDowall Laboratory
Garstang 8.52/8.51
Faculty of biological sciences
University of Leeds
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