[EMAIL PROTECTED] wrote:
> Keith: Thanks for the input. No, I did not have fecals done (even in the 
>metro-Chicago area such vets are hard to find), although I did examine her feces 
>myself at 40X and 100X without any signs of infection.

Ahhh that may be the problem.  40X and 100X is a tich high for fecal work when 
scanning the slide.  Try starting at 10x (some even use 4x), then when you see 
something suspicious, flip to a higher power for fine morphology.  Otherwise you may 
miss significant portions of the slide as you move the slide back and forth

Having said that, there is also the issue of Qx control for the floatation solutions.  
Floatation is a method of concetrating the ova so that you are better able to find 
them.  Comparisons of direct vs float methodologies have shown floats to be far more 
sensitive for most parasites (notice I said most, but not all).  Floatation solutions 
must be watched reasonably carefully, unless you use it quickly as evaporation over 
time can play havok with your ability to float ova and not debris.

In leopard Gex I would strongly reccomend that people ask for acid fast stains on 
fresh feces, particularly in Qx animals, and animals that are losing weight.  More 
expensive yes, but cheaper than de-populating in the face of a crypto outbreak that 
got too far.

Keith

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