Pressure cook the stuff at 15 lbs ofr 15-20 minutes. Presto - sterile media. You can alsmost always find pressure cookers at yard sales for a song and a dance and they are, in fact, simply an autoclave without the fancy gizmos. Aside from the much maligned, but very cute, water bears (tardigrades) - nothing will suvive that trip.

http://www.bioimages.org.uk/HTML/P157312.HTM
http://brainew.com/e/ezboard.cgi/db=alife_debate&action=read&dbf=200312290000
http://www.q7.com/~vvv/tardigrade/
http://www.portlandpress.com/pcs/books/prod_det.cfm?product=1855780437
http://www.tardigrades.com/

Keith


Neil Meister wrote:


Does anyone have a method of debugging fresh-collected sphagnum
moss? It's very hard to buy good quality sphagnum here, but it is
very common locally.

Thanks,
Neil


--
The best servants of the people, like the best valets, must whisper unpleasant truths in the master's ear. It is the court fool, not the foolish courtier, whom the king can least afford to lose - Walter Lippmann



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