Hello, I have been analyzing a particular set of genes for copy number changes that would affect exons. In doing this work, I found some exon size discrepancies between RefSeq sequences and the transcript sequences provided by Ensembl. I have attached the examples that I have found. There are probably more than this. I understand that this may stem from differences in algorithms used for alignment, but I am concerned that this would affect the translated protein sequence because there would be frameshifts occurring. Which transcript is correct? Do we have to wait until more RNA sequencing is done to really know the answer? What about all the libraries of cDNA expression clones that have been made? Would they be at risk of being erroneous? Sincerely, Hannah Cheung, PhD Postdoctoral Associate Department of Pediatrics 1120 Bates St. Feigin Center, Rm 1200.18 Texas Children's Hospital Houston, TX 77030
_______________________________________________ Genome maillist - [email protected] https://lists.soe.ucsc.edu/mailman/listinfo/genome
