I have a collection of ESTs that contain a short target sequence I am
interested in.

Most of them can be matched to chromosome coordinates that have a
matching genome sequence, but several do not, indicating that they are
either mis-sequenced or may be spliced within the target sequence.

Is there a way to query (preferably in batch mode) for the chromosome
coordinates of a sequence within an EST?

 

For example, if an EST has a length of 1000 and I know my target
sequence is at position 408-468 within the EST, but because of introns,
the length of the EST on the chromosome is much longer than 1000.  Is
there a way to structure a query with the EST accession numbers and
target positions and get chromosome coordinates for the targets only? 

 

Thanks

Jim

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