Hello,
I created a few bigWig files from ChIP-seq data aligned to Drosophila
ananassae (droAna1) and Drosophila erecta (droEre1). The files display fine
as custom tracks in the genome browser, but the command line tools give
errors (shown below). We have a number of bigWig files for other Drosophila
species that do not display this problem (including dm3, dp3, droSim1 and
droYak2). The two problem species do have large numbers of scaffolds instead
of chromosome alignments (over 5,000 for erecta and over 11,000 for
ananassae).
Here are the errors reported by bigWigToBedGraph and bigWigInfo (I've
downloaded the latest binary of each):
$ bigWigToBedGraph dan_wce.bw dan_wce.bedgraph
udc couldn't read 1 bytes from dan_wce.bw, did read 0
$ bigWigInfo dan_wce.bw
version: 1
isSwapped: 0
zoomLevels: 7
10
100
1000
10000
100000
1000000
10000000
udc couldn't read 1 bytes from dan_wce.bw, did read 0
Here is an example of a problematic bigWig file:
http://tracks.stowers-institute.org/microarray/repo/2009-09-18_dan_wce_2to4h
_1_101.bw
Thanks for any assistance,
Jeff Johnston
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