Dear Srs. I am making Blat and Blast search with oligonucleotides (25 to 30 pb) against human genome and I have differences in the results. Some oligonucleotides match in a different region to the used for primers design one, when I search with Blat (It does not occur with Blast). Do you know why could it happen? Thank you very much. Dr. Lucas Daurelio.
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