Hi,

Sice my reads are just 32bp long, I am trying to use blat instead of blastx to 
map them into proteins.
I am trying to run
 blat nr.fa seqsaglobus.fa  ris2.psl -t=prot -q=dnax -tileSize=8 -stepSize=3 -
fine -repMatch=1000000 -out=blast8

But I got the message:
d and q must both be either protein or dna

I tried with
-t=prot -q=prot
and I get some results, but it should not work because seqsaglobus.fa is made 
by nucleotides.

Can you explain me what happens?

Thank you,

Fabio

-- 

F. Gori, PhD student
Intelligent Systems
ICIS (Institute for Computing and Information Sciences)
Radboud University Nijmegen

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