Hello,

All of the sequences that considered for alignment are retained in the 
table gbCDnaInfo and gbStatus (even if they do not align).

Sample mySQL query vs gbStatus to get count:

      select count(*) from gbStatus where type = "EST" and orgCat = 
"native" and numAligns = 0;

Modify the query to output IDs (using the public mySQL server) or use 
your own tools versus the text file version after obtaining them from 
the Downloads server (ftp/rsync).

Public mySQL:
http://genome.ucsc.edu/FAQ/FAQdownloads.html#download29

Data downloads:
http://genome.ucsc.edu/FAQ/FAQdownloads.html#download1
http://hgdownload.cse.ucsc.edu/downloads.html
http://genome.ucsc.edu/goldenPath/help/hgTracksHelp.html#Download

For the most recent human and mouse, the data would be here:
http://hgdownload.cse.ucsc.edu/goldenPath/hg19/database/
http://hgdownload.cse.ucsc.edu/goldenPath/mm9/database/

Jennifer

---------------------------------
Jennifer Jackson
UCSC Genome Informatics Group
http://genome.ucsc.edu/

On 3/14/10 11:43 AM, Erez Levanon wrote:
> Hi
>
> Many problematic ESTs and mRNAs are rejected during their alignments to the
> genome (e.g less than 96% base identity with the genomic sequence). I wonder
> where/if can i find a list of those ESTs (in human and mouse) as we are
> interested in such sequences.
>
> Thanks
>
> Erez
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> https://lists.soe.ucsc.edu/mailman/listinfo/genome
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