Hey Saatvik,

I'm able to replicate your error here.  The problem is our support for
tracks of type "maf" don't support the use of the trackDb variable
"mafFile" properly.  A work-around for this problem is to make the
type of your track "wigMaf" in the trackDb entry (see README in
kent/src/hg/makeDb/trackDb for details).

I hope this resolves your error.  If not, please respond to this
message cc'ing the mail list.

brian

On Thu, Mar 18, 2010 at 2:48 AM, Saatvik Agarwal
<[email protected]> wrote:
> Hello,
>
> I am trying to display pairwise alignments generated by BLAT (Genbank
> sequences to hg18) using the MAF format. I like the MAF format since it is
> pretty close to the UCSC cgi custom track for blat results in that it shows
> gaps and mismatches graphically. Now if I upload my MAF track (attached) as
> a custom track, it works fine, but if I load it into our mysql database (we
> run a personal browser mirror) using hgLoadMaf and add it to my trackDb.ra
> entry it doesn't work properly. It looks fine when you're zoomed out but
> when I zoom in (to the point where it would normally show the seq. ident
> plot) the browser crashes and just gives me a completely blank webpage. It's
> obviously this track because if I turn it off everything else works fine.
>
> Any thoughts on what I might be doing wrong? All the examples I saw in the
> kent src doc were multiple alignments to reference genomes. My MAF aligns
> short sequences from Genbank to a reference genome.
>
> My commands for loading the MAF looks like this:
>
> hgLoadMaf hg18 bejsaGenbankBLAT
> -pathPrefix=/cluster/u/curator/tracks/hg18 -custom
> -loadFile=genbankBLAT.maf
> hgLoadMafSummary -minSize=50 -mergeGap=500 -maxSize=50000 hg18
> bejsaGenbankBLATSummary /cluster/u/curator/tracks/hg18/genbankBLAT.maf
>
> and my hg18 trackDb.ra entry is as follows:
>
> track bejsaGenbankBLAT
> shortLabel Endogenous enhancers
> longLabel Endogenous (same species) enhancers BLAT
> color 0,255,0
> priority 1000
> group regulation
> visibility dense
> mafDot on
> type maf
> mafFile /cluster/u/curator/tracks/hg18/genbankBLAT.maf
> summary bejsaGenbankBLATSummary
>
> Thanks,
> Saatvik
>
> _______________________________________________
> Genome maillist  -  [email protected]
> https://lists.soe.ucsc.edu/mailman/listinfo/genome
>
>

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