Hello everyone.
I'm looking for compare affymetrix probe set expression from the different probe cell positions on gene. I downloaded the affymetrix annotation file about HG U133 plus 2 microarray (HG 18 and HG 17) and I converted them into HG 19 model with Lift Over tool. The problem is the folowing: when I compare result with data provided by affymetrix (NetAffx tool), I obtain some times the same data (i.e. probe set 1007_s_at) and sometimes conflicting data (i.e. probe set 1053_at). Furthermore, Genome browser seems to generaly agree whith affymetrix data. I would like to understand why I founded these diferencies and which are the best data to use. I may did something wrong and someone may correct me in my protocol Thanks for your attention Best regards Alexandre _______________________________________________ Genome maillist - [email protected] https://lists.soe.ucsc.edu/mailman/listinfo/genome
