Dear all,

I have downloaded the upstream1000.maf.gz file for the hg19 build using 
the following URL:

http://hgdownload.cse.ucsc.edu/goldenPath/hg19/multiz46way/maf/

This file contains the upstream region of RefSeq genes with annotated 
5'UTRs. I find some inconsistency in this file in comparison to the UCSC 
genome browser annotation:

########################################
refseq-acc: NM_000586

=> extract of the upstream1000.maf file:
...
r txupstream 1000 NM_000586
s hg19.chr4 67775626 1000 - 191154276 AGGACTCTCT-CTGAGACAGG...
...
-----------------------
But the genome browser find this gene in the following location:

NM_000586 at chr4:123372630-123377650


########################################

refseq-acc: NM_000162

=> extract of the upstream1000.maf file:
...
r txupstream 1000 NM_000162
s hg19.chr7 114908641 1000 - 159138663 acctctgag...
...
-----------------------
But the genome browser find this gene in the following location:

NM_000162 at chr7:44183870-44229022
########################################




Did I miss something? Could you please explain me that problem?

Martin Haubrock.

--
Department of Bioinformatics (UMG)
University Medicine Göttingen, Goldschmidtstr. 1, 37075 Göttingen

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