Hello Inbal, When using the BlatBot.pl program, it is important to be certain that it is run only once during any time period and that it fully completes before launching it again with another set of queries.
http://genomewiki.cse.ucsc.edu/index.php/Blat_Scripts Your block will decay in about 12 hours, at which time you can start again. If you would like to share details about how you utility SFmap is using BlatBot.pl, because you feel that the usage criteria has been met and you are still being blocked, we would be interested in learning more. You can send the information to me directly and I will forward to the appropriate staff here for review and feedback. Please do not send anything large in an attachment. If you feel like you need to share data, please set up an ftp location and send access instructions. Thank you, Jennifer --------------------------------- Jennifer Jackson UCSC Genome Informatics Group http://genome.ucsc.edu/ On 4/26/10 6:35 AM, Inbal Paz wrote: > Hi, > > My name is Inbal Paz and I'm a programmer at Prof. Yael Mandel-Gutfreund's > lab located in the Technion- Israel Institute of Technology. We have > recently developed a web-server called 'SFmap' for the mapping and > prediction of splicing factors binding sites (http://sfmap.technion.ac.il/). > The related paper was accepted by Nucleic Acids Res. and is currently under > the final stages of review. > > SFmap uses BLAT in order to determine the genomic coordinates of the input > sequence we get from the user. We run BLAT using the BlatBot.pl script on > the UCSC servers in order to get fast results, since our available hardware > is not strong enough to run BLAT on whole genomes locally in a reasonable > time. > > Yesterday we tried to run SFmap with a long list of input sequences (for QA > purposes) and our web-server's IP was apparently blocked . This is the error > message we got from the BLAT output: > > "There is an exceedingly high volume of traffic coming from your site (IP > address 132.68.104.195) as of Mon Apr 26 03:06:05 2010 (California time). > It looks like a web robot is launching queries quickly, and not even waiting > for the results of one query to finish before launching another query. We > cannot service requests from your IP address under these conditions. (code > 844360)" > > We would be very happy to get the IP released again asap (if this is indeed > the problem) and to get your advise on which is the best way to avoid > similar problems in the future. > > Thanks a lot in advance, > > Inbal > > Inbal Paz > Programmer and Webmaster > Yael Mandel-Gutfreund's lab > Technion - Israel Institute of Technology > Tel: +972-4-8293701 > > > > > > > > _______________________________________________ > Genome maillist - [email protected] > https://lists.soe.ucsc.edu/mailman/listinfo/genome _______________________________________________ Genome maillist - [email protected] https://lists.soe.ucsc.edu/mailman/listinfo/genome
