Hello Elizabeth,

The solution is to give a unique "name" identifier to each element in 
the BED file, rather than giving each the same "name" identifier, for 
your case, a "3".

When I loaded chr22 sample data in the original format with name = "3", 
I experienced the same browser behavior as you did. When I named the 
elements on chrom 22 with identifiers 1-9, the issue was resolved.

I will pass this one to the development team for review since this might 
not be an intentional constraint. Many native browser tracks have 
duplicated names, although they are not in BED format.

As a side issue, you do have coordinates that extend outside of the 
chromosomes actual known sequence, but this does not seem to be an 
issue. Just something to be aware of.

Please try the unique names and see if the problem is eliminated for you 
as well.

Hopefully this helps! Thank you for sending your question along with 
such a clear explanation and with sample data. This was very useful.

Jennifer

---------------------------------
Jennifer Jackson
UCSC Genome Informatics Group
http://genome.ucsc.edu/

On 5/17/10 7:24 AM, Wohler, Elizabeth wrote:
> Hello,
>
> I am experiencing a problem with one of our custom bed file tracks that we 
> have developed. When the track is displayed in the Genome Browser (March 2006 
> build/NCBI36), each bar appears to display correctly. However, when I click 
> on some of the bars, the base pair position that is listed is incorrect for 
> some of the bars. I have attached the custom .bed file for you to look at to 
> see the same problem. For example, on chromosome 22 there are nine bars 
> present on the chromosome. The base pair locations for bars #4 and 8 are 
> incorrect, even though they are listed correctly in the .bed file and are 
> displaying correctly in the Genome Browser. The problem seems to be resolved 
> when I take away the thickStart and thickEND and just use a simplified .bed 
> file, however I would like to continue to use the colored bars and cannot do 
> so when it is in a simplified form. I have a few other custom files that also 
> are showing this same effect, but I have attached this one as an example.
>
> Thank you very much for your assistance,
> Elizabeth Wohler
>
> Kennedy Krieger Institute
> 707 N Broadway
> Baltimore, MD 21205
> (443) 923-2785
>
>
>
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