Thanks Vanessa.

Is it possible to emulate this sort of display with any of the custom 
track formats available?

I have looked into it somewhat and none of the custom track formats seem 
to support multiple colours in a single track line.

Does webBLAT use pslx formatted data for the tracks it displays in the 
Genome Browser? Can custom tracks be in pslx format?

~Thanks,
Lucas

Vanessa Kirkup Swing wrote:
> Dear Lucas,
>
> Unfortunately, we don't support this in psl customTracks because the plain 
> psl does not have the necessary information for a detailed alignment, i.e. 
> where every substitution is.
>
> Please don't hesitate to contact the mailing list if you have further 
> questions.
>
> Vanessa Kirkup Swing
> UCSC Genome Bioinformatics Group
>
>
> ----- Original Message -----
> From: "Lucas Swanson" <[email protected]>
> To: [email protected]
> Sent: Tuesday, July 27, 2010 5:55:28 PM GMT -08:00 US/Canada Pacific
> Subject: [Genome] Custom Tracks Resembling WebBLAT Alignments
>
> Hello,
>
> When webBLAT is used to align a sequence, and then the alignment is 
> viewed in the Genome Browser,
> "alignments of nucleotides use color to highlight differences as follows:
>
>     * *red:* Genome and query sequence have different bases at this
>       position.
>     * *orange:* The query sequence has an insertion (or genome has a
>       deletion / alignment gap) at this point.
>     * *purple:* The query sequence extends beyond the end of the alignment.
>     * *green:* The query sequence appears to have a polyA tail which is
>       not aligned to the genome.
>
> Also, when the alignment has gaps for both genome and query sequence, 
> double horizontal lines are drawn."
>
> However, if command-line BLAT is used to do the alignment, and then the 
> psl output file added to the Genome Browser as a custom track, all of 
> this colour and double-line information is lost.
>
> Is there any way to make a custom track that retains this information in 
> any way?
>
> ~Thanks,
> Lucas Swanson
> _______________________________________________
> Genome maillist  -  [email protected]
> https://lists.soe.ucsc.edu/mailman/listinfo/genome
>   

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