I understand why gfServer requires a perfect match for tileSize + stepSize - 1 bases. But when looking for primers, why does the perfect match have to be at the 3' end, rather than any place else in the primer? (Or is the usage information for gfPcr incorrect?)
TIA, Greg _______________________________________________ Genome maillist - [email protected] https://lists.soe.ucsc.edu/mailman/listinfo/genome
