Dear friends,

I would like to check if a line in BED file belongs to a specific genomic
region (e.g. start = chr1 100; end= chr1 1000). If the line is in reverse
strand, do I need to convert its coordinate present in BED file by the
following formula before checking?

start = (chromSize - lineEnd) + 1
end = chromSize - (lineStart - 1)

Thanks a lot,

Thong

-- 
NGUYEN Trung Thong
School of Mathematics, Statistics and Applied Mathematics
NUI Galway, Ireland
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