Hi Mousheng,

One of my colleagues pointed out that there is a page on our genomewiki 
that describes how to get data values for wiggle tracks from the files 
that are already available via ftp:
http://genomewiki.cse.ucsc.edu/index.php/Using_hgWiggle_without_a_database

You will need the files:
ftp://hgdownload.cse.ucsc.edu/goldenPath/hg17/database/dukeDnaseCd4Signal.txt.gz
ftp://hgdownload.cse.ucsc.edu/gbdb/hg17/wib/dukeDnaseCd4Signal.wib

The "hgWiggle" tool is part of the Genome Browser source code, which is 
free for noncommercial use:
http://hgdownload.cse.ucsc.edu/downloads.html#source_downloads

If you have further questions, please feel free to contact us again at 
[EMAIL PROTECTED]

--
Brooke Rhead
UCSC Genome Bioinformatics Group


On 11/07/08 12:50, mousheng xu wrote:
> Dear Brooke,
> 
> I have the "Duke DNase Sites" track already, but I do need the "Sig"
> track. I would really appreciate it if you could make the "Sig" track
> data available to me. One-score-per-base is fine with me.
> 
> Thanks a lot!
> 
> Sincerely,
> 
> Mousheng Xu
> 
> On Fri, Nov 7, 2008 at 3:30 PM, Brooke Rhead <[EMAIL PROTECTED]> wrote:
>> Hello Mousheng,
>>
>> The "Duke DNase Sig" track in the hg17 database (dukeDnaseCd4Signal) was
>> actually submitted to us as per-base scores.  That is, the data is in
>> fixed-step wiggle format, with 1-base steps.  A description of the wiggle
>> data format is here:
>> http://genome.ucsc.edu/goldenPath/help/wiggle.html
>>
>> If you like, we can make the dukeDnaseCd4Signal wiggle file available to
>> you.  However, it will still be in a one-score-per-base format.
>>
>> You might be interested in using the "Duke DNase Sites" track instead of
>> the "Duke DNase Sig" track.  If not, and if you would still like the signal
>> data in wiggle format, please let us know.
>>
>> --
>> Brooke Rhead
>> UCSC Genome Bioinformatics Group
>>
>>
>> On 11/05/08 10:30, mousheng xu wrote:
>>> Dear UCSC genome help,
>>>
>>> I am trying to get data in "dukeDnaseCd4Signal", but the data are
>>> organized
>>> in a very inefficient way -- signal is recorded for every base instead of
>>> subsequence. For instance, suppose chr1:100-200 has the same signal level
>>> -0.54, instead of giving chr1 100 200 -0.54 as one entry,
>>> "dukeDnaseCd4Signal" gives 101 entries from position 100 to 200 with the
>>> same value -0.54 like below
>>> 100 -0.54
>>> 101 -0.54
>>> ...
>>> 200 -0.54
>>>
>>> The problem is that it unnecessarily takes enormous amount of space.
>>>
>>> Thanks a lot in advance!
>>>
>>> Sincerely,
>>>
>>> Mousheng Xu
>>>
>>> Research Fellow
>>> Brigham & Women's Hospital
>>> Harvard Medical School
>>> 617-525-0739
>>> _______________________________________________
>>> Genome maillist  -  [email protected]
>>> http://www.soe.ucsc.edu/mailman/listinfo/genome
_______________________________________________
Genome maillist  -  [email protected]
http://www.soe.ucsc.edu/mailman/listinfo/genome

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