Hi UCSC,
I was wondering about why blat lists negative values for the tStart/End in
this psl result.
I was aligning an amino acid sequence against PDB records:
103 0 0 0 0 0 0 0 +
NP_005347.3 509 123 226 1BHH 108 -152 -49 1
103, 123, -152,
The command I ran was:
blat Human2.5Updated.fa RefSeqAA.fa -prot -out=pslx -minIdentity=100
blat100output.pslx
Have you seen this before? I'm a bit confused, so any help would be great.
Thanks!
Amit
Amit R. Indap
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