Hello,

Apologies for the multiple emails, I am still trying to figure out how
stranding of the mRNAs is determined in the genome browser. More
specifically, how is stranding determined for those mRNAs that have a 0 in
the mrnaOrientInfo table?

Thank you for your help,

Nandita






On Thu, Jan 15, 2009 at 2:46 PM, Nandita Garud <[email protected]> wrote:

> Hello,
>
> I am trying to understand how strand information for mRNAs in the all_mrna
> table is determined. I have followed the FAQ about estOrientInfo, and am I
> correct in understanding that if the intronOrientation field is negative,
> then the strand orientation should actually be opposite of what is listed in
> the all_est table? Is this the same for the mRNAs? How is orientation in the
> all_mrna table determined in the first place? Is it based on the orientation
> suggested by the one who submitted the mRNA to the database, but then
> possibly revised in the mrnaOrientInfo table? If the intronOrientation is 0
> in the mrnaOrientInfo table, how probable would it be that the orientation
> suggested by the all_mrna table is correct?
>
> Thank you,
>
> Nandita
>
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